ZNRF2 Polyclonal Antibody
- 货号：YT4986
- 应用：IHC;IF;ELISA
- 种属：Human;Mouse
- 蛋白名称：
- E3 ubiquitin-protein ligase ZNRF2
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human ZNRF2. AA range:161-210
- 特异性：
- ZNRF2 Polyclonal Antibody detects endogenous levels of ZNRF2 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- ZNRF2;RNF202;E3 ubiquitin-protein ligase ZNRF2;Protein Ells2;RING finger protein 202;Zinc/RING finger protein 2
- 背景：
- domain:The RING-type zinc finger domain is required for E3 ligase activity.,function:May play a role in the establisment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 RING-type zinc finger.,subcellular location:Present in presynaptic plasma membranes in neurons.,subunit:Interacts with UBE2N.,tissue specificity:Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney.,
- 功能：
- domain:The RING-type zinc finger domain is required for E3 ligase activity.,function:May play a role in the establisment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 RING-type zinc finger.,subcellular location:Present in presynaptic plasma membranes in neurons.,subunit:Interacts with UBE2N.,tissue specificity:Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney.,
- 细胞定位：
- Endosome membrane ; Peripheral membrane protein . Lysosome membrane ; Peripheral membrane protein . Cell junction, synapse, presynaptic cell membrane ; Peripheral membrane protein .
- 组织表达：
- Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney.
- Immunofluorescence analysis of A549 cells, using ZNRF2 Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
