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V-ATPase D Polyclonal Antibody

货号：YT4860
应用：WB;ELISA
种属：Human;Mouse;Rat;Swine

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靶点：
V-ATPase D

简介：
>>Oxidative phosphorylation;>>Metabolic pathways;>>Phagosome;>>mTOR signaling pathway;>>Synaptic vesicle cycle;>>Collecting duct acid secretion;>>Vibrio cholerae infection;>>Epithelial cell signaling in Helicobacter pylori infection;>>Human papillomavirus infection;>>Rheumatoid arthritis

基因名称：
ATP6V1D

蛋白名称：
V-type proton ATPase subunit D

Human Gene Id：
51382

Human Swiss Prot No：
Q9Y5K8

Mouse Gene Id：
73834

Mouse Swiss Prot No：
P57746

免疫原：
Synthesized peptide derived from V-ATPase D . at AA range: 70-150

特异性：
V-ATPase D Polyclonal Antibody detects endogenous levels of V-ATPase D protein.

组成：
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

来源：
Polyclonal, Rabbit,IgG

稀释：
WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.

纯化工艺：
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

浓度：
1 mg/ml

储存：
-15°C to -25°C/1 year(Do not lower than -25°C)

其他名称：
ATP6V1D;ATP6M;VATD;V-type proton ATPase subunit D;V-ATPase subunit D;V-ATPase 28 kDa accessory protein;Vacuolar proton pump subunit D

实测条带：
28kD

背景：
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the V1 domain D subunit protein. [provided by RefSeq, Jul 2008],

功能：
function:Subunit of the peripheral V1 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.,similarity:Belongs to the V-ATPase D subunit family.,subunit:V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d).,

细胞定位：
Membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasmic vesicle, clathrin-coated vesicle membrane ; Peripheral membrane protein . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cell projection, cilium . Localizes to centrosome and the base of the cilium. .

组织表达：
Bone marrow,Brain,Heart,Pancreatic adenocarcinoma,Pituitary,Placent

Autophagy flux inhibition mediated by lysosomal dysfunction participates in the cadmium exposure-induced cardiotoxicity in swine 2022 Mar 14. WB Swine 1:1000 myocardium
货号：YT4860