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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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EAR2 Polyclonal Antibody
- 货号:YT1450
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Nuclear receptor subfamily 2 group F member 6
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human NR2F6. AA range:11-60
- 特异性:
- EAR2 Polyclonal Antibody detects endogenous levels of EAR2 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- NR2F6;EAR2;ERBAL2;Nuclear receptor subfamily 2 group F member 6;V-erbA-related protein 2;EAR-2
- 背景:
- similarity:Belongs to the nuclear hormone receptor family. NR2 subfamily.,similarity:Contains 1 nuclear receptor DNA-binding domain.,
- 功能:
- similarity:Belongs to the nuclear hormone receptor family. NR2 subfamily.,similarity:Contains 1 nuclear receptor DNA-binding domain.,
- 组织表达:
- Expressed in heart, placenta, liver, skeletal muscle, kidney and pancreas.
- Western Blot analysis of various cells using EAR2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunofluorescence analysis of HepG2 cells, using NR2F6 Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma tissue, using NR2F6 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HeLa, HUVEC, and 293 cells, using NR2F6 Antibody. The lane on the right is blocked with the synthesized peptide.