CDC46 Polyclonal Antibody
- 货号：YT0812
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse;Rat
- 简介：
- >>DNA replication;>>Cell cycle
- 蛋白名称：
- DNA replication licensing factor MCM5
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human MCM5. AA range:21-70
- 特异性：
- CDC46 Polyclonal Antibody detects endogenous levels of CDC46 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- MCM5;CDC46;DNA replication licensing factor MCM5;CDC46 homolog;P1-CDC46
- 背景：
- The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation. [provided by RefSeq, Jul 2008],
- 功能：
- similarity:Belongs to the MCM family.,similarity:Contains 1 MCM domain.,
- 细胞定位：
- Nucleus . Chromosome . Cytoplasm, cytosol . Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses. .
- 组织表达：
- Cervix,Cervix carcinoma,Lymph,Placenta,Spleen,Thymus,Tongue
- Western Blot analysis of various cells using CDC46 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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- Western Blot analysis of HepG2 cells using CDC46 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunofluorescence analysis of HepG2 cells, using MCM5 Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using MCM5 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HepG2 cells, using MCM5 Antibody. The lane on the right is blocked with the synthesized peptide.
