ATRIP Polyclonal Antibody
- 货号：YT0417
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse;Rat
- 简介：
- >>Fanconi anemia pathway
- 蛋白名称：
- ATR-interacting protein
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human ATRIP. AA range:34-83
- 特异性：
- ATRIP Polyclonal Antibody detects endogenous levels of ATRIP protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- ATRIP;AGS1;ATR-interacting protein;ATM and Rad3-related-interacting protein
- 背景：
- This gene encodes an essential component of the DNA damage checkpoint. The encoded protein binds to single-stranded DNA coated with replication protein A. The protein also interacts with the ataxia telangiectasia and Rad3 related protein kinase, resulting in its accumulation at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2012],
- 功能：
- caution:The gene for this protein is either identical to or adjacent to that of TREX1. Some of the mRNAs that encode ATRIP also encode TREX1 in another reading frame.,domain:The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.,function:Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.,PTM:Phosphorylated by ATR.,sequence caution:Translation N-terminally extended.,similarity:Belongs to the ATRIP family.,subcellular location:Redistributes to discrete nuclear foci upon DNA damage.,subunit:Heterodimer with ATR. The heterodimer binds the RPA complex and is then recruited to single stranded DNA. Interacts with CEP164 (via N-terminus).,tissue specificity:Ubiquitous.,
- 细胞定位：
- Nucleus . Redistributes to discrete nuclear foci upon DNA damage.
- Western Blot analysis of various cells using ATRIP Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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- Western Blot analysis of 3T3 cells using ATRIP Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunofluorescence analysis of HeLa cells, using ATRIP Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using ATRIP Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from NIH/3T3 cells, using ATRIP Antibody. The lane on the right is blocked with the synthesized peptide.
