ARP-1 Polyclonal Antibody
- 货号:YT0335
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- COUP transcription factor 2
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human COT2. AA range:1-50
- 特异性:
- ARP-1 Polyclonal Antibody detects endogenous levels of ARP-1 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- NR2F2;ARP1;TFCOUP2;COUP transcription factor 2;COUP-TF2;Apolipoprotein A-I regulatory protein 1;ARP-1;COUP transcription factor II;COUP-TF II;Nuclear receptor subfamily 2 group F member 2
- 背景:
- This gene encodes a member of the steroid thyroid hormone superfamily of nuclear receptors. The encoded protein is a ligand inducible transcription factor that is involved in the regulation of many different genes. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010],
- 功能:
- function:Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A.,similarity:Belongs to the nuclear hormone receptor family.,similarity:Belongs to the nuclear hormone receptor family. NR2 subfamily.,similarity:Contains 1 nuclear receptor DNA-binding domain.,subunit:Interacts with SQSTM1 (By similarity). Binds DNA as a homodimer.,tissue specificity:Found in all tissues.,
- 组织表达:
- Ubiquitous. Expressed in the stromal cells of developing fetal ovaries (PubMed:29478779).
- Western Blot analysis of various cells using ARP-1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Immunofluorescence analysis of HUVEC cells, using COT2 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HUVEC cells, using COT2 Antibody. The lane on the right is blocked with the synthesized peptide.