GRK 2 (phospho Ser685) Polyclonal Antibody
- 货号：YP0456
- 应用：WB;ELISA
- 种属：Human;Mouse;Rat
- 简介：
- >>Chemokine signaling pathway;>>Endocytosis;>>Hedgehog signaling pathway;>>Glutamatergic synapse;>>Olfactory transduction;>>Morphine addiction
- 蛋白名称：
- Beta-adrenergic receptor kinase 1
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human GRK2 around the phosphorylation site of Ser685. AA range:640-689
- 特异性：
- Phospho-GRK 2 (S685) Polyclonal Antibody detects endogenous levels of GRK 2 protein only when phosphorylated at S685.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- ADRBK1;BARK;BARK1;GRK2;Beta-adrenergic receptor kinase 1;Beta-ARK-1;G-protein coupled receptor kinase 2
- 背景：
- The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq, Jul 2008],
- 功能：
- catalytic activity:ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate.,catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them.,online information:Beta adrenergic receptor kinase entry,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 RGS domain.,subunit:Interacts with GIT1 (By similarity). Interacts with, and phosphorylates chemokine-stimulated CCR5.,tissue specificity:Expressed in peripheral blood leukocytes.,
- 细胞定位：
- Cytoplasm . Cell membrane . Cell junction, synapse, postsynapse . Cell junction, synapse, presynapse .
- 组织表达：
- Expressed in peripheral blood leukocytes.
- Western Blot analysis of JK cells using Phospho-GRK 2 (S685) Polyclonal Antibody diluted at 1:500
- Western blot analysis of lysates from HT29 cells treated with insulin 0.01U/ml 15', using GRK2 (Phospho-Ser685) Antibody. The lane on the right is blocked with the phospho peptide.
