His Tag (PT0481R) PT® Rabbit mAb

    • 货号:YM8314
    • 应用:WB;IF;IP;ELISA
      • 靶点:
      • His-Tag
      • 基因名称:
      • His-Tag
      • 蛋白名称:
      • His Tag
      • 特异性:
      • endogenous
      • 组成:
      • PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
      • 来源:
      • Monoclonal, rabbit, IgG, Kappa
      • 稀释:
      • WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;
      • 纯化工艺:
      • Protein A
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 分子量:
      • 50kD
      • 实测条带:
      • 55kD
      • 背景:
      • A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

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      • 产品图片
      • Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-His Tag (PT0481R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Flag-His-MBP Lane 2: Myc-His-MBP Lane 3: HA-His-MBP Predicted band size: 50kDa Observed band size: 55kDa