- 靶点:
- His-Tag
- 基因名称:
- His-Tag
- 蛋白名称:
- His Tag
- 特异性:
- endogenous
- 组成:
- PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
- 来源:
- Monoclonal, rabbit, IgG, Kappa
- 稀释:
- WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;
- 纯化工艺:
- Protein A
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 分子量:
- 50kD
- 实测条带:
- 55kD
- 背景:
- A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
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货号:YM8314
Development and characterization of in vitro self-assembled recombinant human follicle stimulating hormone originated from goat mammary epithelial cells. MOLECULAR AND CELLULAR ENDOCRINOLOGY Mol Cell Endocrinol. 2021 Apr;526:111211 Pull down Hamster 1:16000 Chinese hamster ovary (CHO) cell
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Betulin efficiently suppresses the process of an experimental Listeria monocytogenes infection as an antagonist against listeriolysin O. FITOTERAPIA Fitoterapia. 2019 Nov;139:104409 WB Listeria monocytogenes
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- 产品图片
- Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-His Tag (PT0481R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Flag-His-MBP Lane 2: Myc-His-MBP Lane 3: HA-His-MBP Predicted band size: 50kDa Observed band size: 55kDa