• 靶点：
• OPN

• 简介：
• >>PI3K-Akt signaling pathway;>>Apelin signaling pathway;>>Focal adhesion;>>ECM-receptor interaction;>>Toll-like receptor signaling pathway;>>GnRH secretion;>>Human papillomavirus infection

• 基因名称：
• SPP1

• 蛋白名称：
• Osteopontin

• Human Gene Id：
• 6696

• Human Swiss Prot No：
• P10451

• Mouse Gene Id：
• 20750

• Mouse Swiss Prot No：
• P10923

• Rat Gene Id：
• 25353

• Rat Swiss Prot No：
• P08721

• 特异性：
• endogenous

• 组成：
• PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

• 来源：
• Monoclonal, rabbit, IgG, Kappa

• 稀释：
• IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;

• 纯化工艺：
• Protein A

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• SPP1;BNSP;OPN;PSEC0156;Osteopontin;Bone sialoprotein 1;Nephropontin;Secreted phosphoprotein 1;SPP-1;Urinary stone protein;Uropontin

• 分子量：
• 35kD

• 实测条带：
• 55,65kD

• 背景：
• secreted phosphoprotein 1(SPP1) Homo sapiens The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011],

• 功能：
• disease:This protein plays a principal role in urinary stone formation as the stone matrix.,function:Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.,function:Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.,online information:Osteopontin entry,PTM:Extensively phosphorylated on clustered serine residues.,PTM:N- and O-glycosylated.,similarity:Belongs to the osteopontin family.,subunit:Ligand for integrin alpha-V/beta-3.,

• 细胞定位：
• Secreted

• 组织表达：
• Bone. Found in plasma.

CD137-CD137L Aggravates Calcification of Vascular Smooth Muscle Cell and Vasculature of ApoE−/− Mice Via Rab7-Mediated Autophagy IF Mouse 1:200 /VSMCs
货号：YM8281

In vitro effects of recombinant adenovirus-mediated bone morphogenetic protein 2/vascular endothelial growth factor 165 on osteogenic differentiation of bone marrow mesenchymal stem cells. Artificial Cells Nanomedicine and Biotechnology 2016 Jan 13 WB Mouse 1:500 C3H10T1/2 cell
货号：YM8281

Oral Hydrogen-Rich Water Alleviates Oxalate-Induced Kidney Injury by Suppressing Oxidative Stress, Inflammation, and Fibrosis. Frontiers in Medicine Front Med-Lausanne. 2021; 8: 713536 IHC Mouse 1:100 Kidney
货号：YM8281

Elevated c-fos expression is correlated with phenotypic switching of human vascular smooth muscle cells derived from lower limb venous varicosities. Journal of Vascular Surgery-Venous and Lymphatic Disorders J Vasc Surg-Venous L. 2021 Jan;9:242 WB Human 1:1000 VSMCs
货号：YM8281

4D Printing of Personalized-Tunable Biomimetic Periosteum with Anisotropic Microstructure for Accelerated Vascularization and Bone Healing. Mengfei Yu WB Human human mesenchymal stem cells (hMSCs)
货号：YM8281

Increased abundance of bacteria of the family Muribaculaceae achieved by fecal microbiome transplantation correlates with the inhibition of kidney calcium oxalate stone deposition in experimental rats. Pan TieJun IHC Rat kidney tissue
货号：YM8281

Hub gene ELK3-mediated reprogramming lipid metabolism regulates phenotypic switching of pulmonary artery smooth muscle cells to develop pulmonary arterial hypertension induced by PM2.5 JOURNAL OF HAZARDOUS MATERIALS Qingping Liu WB Mouse 1:1000 right ventricle tissue
货号：YM8281

Potential crosstalk between SPP1 + TAMs and CD8 + exhausted T cells promotes an immunosuppressive environment in gastric metastatic cancer Journal of Translational Medicine Du Yan IF Mouse MFC cell-Xenograft
货号：YM8281

Maresin-1 ameliorates hypertensive vascular remodeling through its receptor LGR6 MedComm Zheng Yin IF,WB Mouse,Rat aorta tissue rat aortic smooth muscle cells (RASMCs)
货号：YM8281

Epigallocatechin gallate protects MC3T3-E1 cells from cadmium-induced apoptosis and dysfunction via modulating PI3K/AKT/mTOR and Nrf2/HO-1 pathways PeerJ Fanhao Wei WB Mouse MC3T3-E1 cell
货号：YM8281

Mussel-Derived and Bioclickable Peptide Mimic for Enhanced Interfacial Osseointegration via Synergistic Immunomodulation and Vascularized Bone Regeneration Advanced Science Wei Zhou IHC,WB Rat femur tissue bone marrow mesenchymal stem cell(BMSC)
货号：YM8281

Naringenin modulates the NO‑cGMP‑PKG signaling pathway by binding to AKT to enhance osteogenic differentiation in hPDLSCs INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Shenghong Li WB,IF Human,Rat 1:2000,1:100 periodontal ligament tissue,new bone tissue human periodontal ligament stem cell(hPDLSC)
货号：YM8281

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