RFP-Tag Monoclonal Antibody(5G4)
- 货号：YM3011
- 应用：WB;IF;
- 种属：Species independent
- 免疫原：
- Recombinant Protein of RFP-Tag
- 特异性：
- The antibody detects RFP ERFP tag fusion proteins. It isn't reactive against GFP CFP YFP. It is reactive against morange tag weakly.
- 组成：
- PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
- 稀释：
- WB 1:10000-20000,IF 1:200-2000
- 纯化工艺：
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景：
- RFP (Red Fluorescent Protein), cloned from Discosoma coral (DsRed, DsRed2 or drFP583), is a protein which exhibits bright red fluorescence when exposed to blue light. It is common research practice for biologists to introduce a gene (or a gene chimera) encoding an engineered fluorescent protein into living cells and subsequently visualize the location and dynamics of the gene product using fluorescence microscopy.
- 1ug RFP fusion protein+ Primary antibody dilution at
1) 1:3000
2) 1:5000
3) 1:10000
