Hox-B9 Polyclonal Antibody
- Catalog No.：YT2217
- Applications：WB;IHC;IF;ELISA
- Reactivity：Human;Mouse
- Target：
- Hox-B9
- Gene Name：
- HOXB9
- Protein Name：
- Homeobox protein Hox-B9
- Human Gene Id：
- 3219
- Human Swiss Prot No：
- P17482
- Mouse Gene Id：
- 15417
- Mouse Swiss Prot No：
- P20615
- Immunogen：
- The antiserum was produced against synthesized peptide derived from human HOXB9. AA range:201-250
- Specificity：
- Hox-B9 Polyclonal Antibody detects endogenous levels of Hox-B9 protein.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Polyclonal, Rabbit,IgG
- Dilution：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- Purification：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name：
- HOXB9;HOX2E;Homeobox protein Hox-B9;Homeobox protein Hox-2.5;Homeobox protein Hox-2E
- Observed Band(KD)：
- 28kD
- Background：
- This gene is a member of the Abd-B homeobox family and encodes a protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded nuclear protein functions as a sequence-specific transcription factor that is involved in cell proliferation and differentiation. Increased expression of this gene is associated with some cases of leukemia, prostate cancer and lung cancer. [provided by RefSeq, Jul 2008],
- Function：
- developmental stage:Expressed in whole embryos and fetuses at 5-9 weeks from conception.,function:Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.,similarity:Belongs to the Abd-B homeobox family.,similarity:Contains 1 homeobox DNA-binding domain.,
- Subcellular Location：
- Nucleus.
- Expression：
- Muscle,Placenta,
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- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using Hox-B9 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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- Western Blot analysis of Hela cells using Hox-B9 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western blot analysis of lysates from K562 cells, using HOXB9 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
