ERGIC-3 Polyclonal Antibody
- Catalog No.:YT1620
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse
- Target:
- ERGIC-3
- Gene Name:
- ERGIC3
- Protein Name:
- Endoplasmic reticulum-Golgi intermediate compartment protein 3
- Human Gene Id:
- 51614
- Human Swiss Prot No:
- Q9Y282
- Mouse Gene Id:
- 66366
- Mouse Swiss Prot No:
- Q9CQE7
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human ERGI3. AA range:321-370
- Specificity:
- ERGIC-3 Polyclonal Antibody detects endogenous levels of ERGIC-3 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- ERGIC3;C20orf47;ERV46;SDBCAG84;CGI-54;Endoplasmic reticulum-Golgi intermediate compartment protein 3;Serologically defined breast cancer antigen NY-BR-84
- Observed Band(KD):
- 43kD
- Background:
- function:Possible role in transport between endoplasmic reticulum and Golgi.,similarity:Belongs to the ERGIC family.,subcellular location:Cycles between the endoplasmic reticulum and the Golgi.,subunit:Interacts with ERGIC1/ERGIC32.,
- Function:
- function:Possible role in transport between endoplasmic reticulum and Golgi.,similarity:Belongs to the ERGIC family.,subcellular location:Cycles between the endoplasmic reticulum and the Golgi.,subunit:Interacts with ERGIC1/ERGIC32.,
- Subcellular Location:
- Endoplasmic reticulum-Golgi intermediate compartment membrane ; Multi-pass membrane protein . Golgi apparatus, cis-Golgi network membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Cycles between the endoplasmic reticulum and the Golgi.
- Expression:
- Brain,Embryo,Fetal liver,Liver,Mammary gland,Skin,
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- Antibody-FAQs
- Products Images
- Immunofluorescence analysis of HepG2 cells, using ERGI3 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from LOVO and NIH/3T3 cells, using ERGI3 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).