• Target：
• Cdk8

• Gene Name：
• CDK8

• Protein Name：
• Cyclin-dependent kinase 8

• Human Gene Id：
• 1024

• Human Swiss Prot No：
• P49336

• Mouse Gene Id：
• 264064

• Mouse Swiss Prot No：
• Q8R3L8

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human CDK8. AA range:1-50

• Specificity：
• Cdk8 Polyclonal Antibody detects endogenous levels of Cdk8 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• CDK8;Cyclin-dependent kinase 8;Cell division protein kinase 8;Mediator complex subunit CDK8;Mediator of RNA polymerase II transcription subunit CDK8;Protein kinase K35

• Observed Band(KD)：
• 53kD

• Background：
• cyclin dependent kinase 8(CDK8) Homo sapiens The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.,catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading

• Subcellular Location：
• Nucleus .

• Expression：
• Testis,

Corosolic acid inhibits cancer progression by decreasing the level of CDK19-mediated O-GlcNAcylation in liver cancer cells. Cell Death & Disease Cell Death Dis. 2021 Sep;12(10):1-11 WB Human 1:500 Bel-7402 cell，Bel-7404 cell

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