KIF20A (phospho Ser528) Polyclonal Antibody
- Catalog No.:YP0373
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Rat;Mouse;
- Target:
- KIF20A
- Gene Name:
- KIF20A
- Protein Name:
- Kinesin-like protein KIF20A
- Human Gene Id:
- 10112
- Human Swiss Prot No:
- O95235
- Mouse Swiss Prot No:
- P97329
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human KIF20A around the phosphorylation site of Ser528. AA range:501-550
- Specificity:
- Phospho-KIF20A (S528) Polyclonal Antibody detects endogenous levels of KIF20A protein only when phosphorylated at S528.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- KIF20A;MKLP2;RAB6KIFL;Kinesin-like protein KIF20A;GG10_2;Mitotic kinesin-like protein 2;MKlp2;Rab6-interacting kinesin-like protein;Rabkinesin-6
- Observed Band(KD):
- 100kD
- Background:
- function:Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.,similarity:Belongs to the kinesin-like protein family.,similarity:Contains 1 kinesin-motor domain.,
- Function:
- function:Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.,similarity:Belongs to the kinesin-like protein family.,similarity:Contains 1 kinesin-motor domain.,
- Subcellular Location:
- Golgi apparatus . Cytoplasm, cytoskeleton, spindle . Localizes to the spindle midzone during anaphase and telophase. .
- Expression:
- Brain,Endothelial cell,Epithelium,Uterus,
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- May 20-2022
- Cell-Based ELISA│解您多样本WB检测之困扰
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Western Blot analysis of 293 cells using Phospho-KIF20A (S528) Polyclonal Antibody
- Western blot analysis of lysates from 293 cells, using KIF20A (Phospho-Ser528) Antibody. The lane on the right is blocked with the phospho peptide.
- Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).