• Target：
• Cofilin

• Fields：
• >>Axon guidance;>>Fc gamma R-mediated phagocytosis;>>Regulation of actin cytoskeleton;>>Pertussis;>>Human immunodeficiency virus 1 infection

• Gene Name：
• CFL1

• Protein Name：
• Cofilin-1

• Human Gene Id：
• 3716

• Human Swiss Prot No：
• P23528

• Mouse Gene Id：
• 12631

• Mouse Swiss Prot No：
• P18760

• Rat Gene Id：
• 29271

• Rat Swiss Prot No：
• P45592

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human Cofilin around the phosphorylation site of Ser3. AA range:1-50

• Specificity：
• Phospho-Cofilin (S3) Polyclonal Antibody detects endogenous levels of Cofilin protein only when phosphorylated at S3.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• CFL1;CFL;Cofilin-1;18 kDa phosphoprotein;p18;Cofilin; non-muscle isoform

• Observed Band(KD)：
• 19kD

• Background：
• cofilin 1(CFL1) Homo sapiens The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004],

• Function：
• function:Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.,online information:Cofilin entry,PTM:Phosphorylated on Ser-3 in resting cells.,similarity:Belongs to the actin-binding proteins ADF family.,similarity:Contains 1 ADF-H domain.,subcellular location:Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.,tissue specificity:Widely distributed in various tissues.,

• Subcellular Location：
• Nucleus matrix . Cytoplasm, cytoskeleton . Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, lamellipodium membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, lamellipodium . Cell projection, growth cone . Cell projection, axon . Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.

• Expression：
• Widely distributed in various tissues.

miR-29a/b/c function as invasion suppressors for gliomas by targeting CDC42 and predict the prognosis of patients. BRITISH JOURNAL OF CANCER Brit J Cancer. 2017 Aug;117(7):1036-1047 WB Human U87MG cell, U251 cell

Downregulation of glycine decarboxylase enhanced cofilin-mediated migration in hepatocellular carcinoma cells. FREE RADICAL BIOLOGY AND MEDICINE Free Radical Bio Med. 2018 May;120:1 WB Human MHCC97L cell, HCCLM3 cell,PLC cell, Hep3B cell

Eucalyptal A inhibits glioma by rectifying oncogenic splicing of MYO1B mRNA via suppressing SRSF1 expression. EUROPEAN JOURNAL OF PHARMACOLOGY Eur J Pharmacol. 2021 Jan;890:173669 WB Human LN229 cell,U87MG cell

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